g/mL s km g cm3 mm mg L Note that the P200 officially has a range from 50-200μl. Never invert or lay down the micropipettor with liquid in the tip. Learn more about accessibility on the OpenLab, © New York City College of Technology | City University of New York, Nucleic Acids: DNA extraction and Dische’s Diphenylamine test (Activity), Quantitative Detection of Protein (activity), Quantitative Detection of Protein (SpectroVis Plus), Do Larger Things Diffuse Faster? Historically, many different systems of units have been used, where a system of units is defined as a collection of units of measurement with rules that relate them to each other. In this case, the value is smaller by 1,000X. The number 0.4382251 rounded to the nearest 0.01 is 0.44. You must first zero the scale before weighing the penny. This weakens the seal on the o-rings and can damage them. Notice that the 2 in 0.526 is rounded up to 3 (0.53) because the digit to the right of the 2 is 6. The metric system is an internationally agreed-upon measurement system based on decimals or powers of 10. The LibreTexts libraries are Powered by MindTouch® and are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. It has a sensitivity of 0.001 g. Due to its sensitivity, moving air will cause it to fluctuate. Record your measurement in 1) millimeters, 2) centimeters, and 3) meters. Remove the beaker from the scale and weigh the penny without using the beaker. Pipet 10 μl in triplicate from each well of the mixing plate into the center of the appropriate circles on the target card. In biology, you will often find a need to describe measurements of length, volume, mass, time, temperature or amount of substance. We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. For example, the number 0.526 becomes 0.5. Learning to correctly use one type of pipettor will provide you the knowledge to use others as they share the same method of distributing small volumes. Metric to English: 1 meter = 39.37008 inches = 3.28084 feet, English to Metric: 1 inch = 0.0254 meters; 1 foot = 0.3048 meters. Metric conversions are done by moving the decimal point. 37) Measure and record the temperature of the air in the laboratory room. The site also includes a predictive tool that suggests possible conversions based on input, allowing for easier navigation while learning more about various unit … The temperature in Fahrenheit can be converted to Celsius using the formula: For example, to convert 60° F to ° C, subtract 32 (=28), multiply it by 5 (=140) and divide it by 9 (=15.56). The unit for volume is the liter (L). The unit for mass is the gram (g). Depress the plunger in destination tube to the first stop. Using the idea of factors of ten, you can assess the difference between the two units and cancel out the original unit algebraically to reach the desired final unit. Subtract the two numbers: (-2 - (-6) = 4). 40) 72° F = _____°C For this one, use the formula \(\mathrm{°C = \dfrac{5}{9}(°F - 32)}\). This lab will illustrate the Rainin Pipetman® micropipettors. Exponents are useful when writing numbers that are very large or very small. 27) 3 oz = _____ g Round your answer to the nearest 0.01 g. 28) Obtain a 10 ml graduated cylinder (shown below) and add some water to it. 38) Measure and record the temperature of ice water. The blank line in the middle of the conversion chart can change depending on what we are measuring: The unit for length is the meter (m). Instruments have a finite amount of accuracy and it is important to report measurements within that level of accuracy. What type of measurement is indicated by each of the following units? Just as a pair of shoes equals 2 shoes, a mole of shoes is 6.022×10, Just as a dozen eggs equals 12 eggs, a mole of eggs is 6.022×10. The metric system is an internationally agreed upon measurement system based on decimals or powers of 10. Unless otherwise noted, LibreTexts content is licensed by CC BY-NC-SA 3.0. 36) 0.5 gallons = _____ liters Round your answer to the nearest 0.01 liter. Measurements are on target. It is not 12.3 feet.]. The tables below show similar units based on grams (mass) and liters (volume). The four pipettor sizes (P10, P20, P200, P1000) used in our lab will measure from 1 μL – 1000 μL as shown below. 22) How much does the penny weigh in grams? An exception to this rule is the P200 labeled 50-200 μl. Pipettors are made by many different manufacturers and thus all do not look the same. Similarly, rounding a number to the nearest 0.01 means that your answer should display two digits to the right of the decimal point. In other words, the value is 1/1000 or 0.001 the value. The table below shows the standard unit of length, mass, volume, and temperature in the metric system. This illustrates precision. Prepare seven dye mixtures as illustrated in the table below. Legal. This illustrates accuracy. Just as a pair of shoes equals 2 shoes, a mole of shoes is 6.022×10, Just as a dozen eggs equals 12 eggs, a mole of eggs is 6.022×10. One meter = 100 cm. They are essential for your success in this course and shared amongst numerous students. Watch the recordings here on Youtube! Practical Molecular Biology. 29) Use a 50 or 100 ml graduated cylinder to determine the amount of liquid that a test tube can hold (it's volume). Accuracy refers to how closely a measured value agrees with the correct or target value. Never measure higher or lower than the range of the pipettor allows. (Note- If you do not have a calculator, use the one on the computer. https://www.youtube.com/watch?v=uEy_NGDfo_8. which states 1000 milliliter in every 1 liter, pay attention to the units and how we’ve canceled out the ml in the numerator of 500ml and in the denominator in the conversion of 1L in 1000ml. Watch the recordings here on Youtube! Missed the LibreFest? The metric system is an internationally agreed-upon measurement system based on decimals or powers of 10.Scientists use a refined version called the International System of Units (abbreviated SI).In biology, you will often find a need to describe measurements of length, volume, mass, time, temperature or amount of substance. 2.6 cm = 26000, The exponent for um is -6. These are precision instruments which can easily be damaged. What is the volume of water in your cylinder? Measurements in science use metric units. The number 0.4382251 rounded to the nearest 0.0001 is 0.4382. Whil… Several of the questions in this exercise ask you to round your answers. Any other quantity of that same kind can be expressed as a multiple of the unit of measurement. The negative sign indicates that you must move the decimal point 4 places to the left. 39) Measure and record the temperature of boiling water. How did you determine the volume of the test tube? The 90 cm mark can be seen in the center of the photograph. So, I wanted to take a moment to share a bit more about units, along with some of the key conversion factors your students should memorize. All of the measurements done in this course will use the metric system. Depress to the second stop if fluid remains in the tip to expel full volume. Carefully draw the plunger up slowly and follow the liquid to avoid drawing air. You must subtract the exponents in order to determine how many places to move the decimal point. Have questions or comments? New York City College of Technology | City University of New York. The metric system is the world standard for measurement. Which unit is larger? Pipettors are more accurate in the upper part of their range. The photograph below shows the end of a meter stick. The “P-number” represents the maximum volume in μL that the pipettor can measure. If the number to the right of the last digit being displayed is 5 or greater, the displayed number is increased by 1. Missed the LibreFest? Significant figures, report the number of digits that are known to some degree of confidence with the measuring device. Treat them with respect and care.